Human blood is an important component of human life, and there are no alternatives to blood components yet. Blood donation is one of the most essential need to manage patients suffering from various medical conditions. There is a great need to create awareness among the population at large and students about blood donation to maintain a regular blood supply. The present study aimed to assess the knowledge of Paramedical Ist year students regarding blood donation. Objectives: assess the pre-test and post-test knowledge regarding blood donation. Materials and methods: Quantitative Pre-experimental one group pre-test post-test design, non-Probability purposive sampling technique was used to select 62 subjects. The data was collected by using self-structured knowledge questionnaire. The result based on the objective showed that change in the post-test knowledge score of subjects was significantly higher than pre-test score. Planned teaching program is proved an effective method to improve knowledge of subjects regarding blood donation. The study also states there was significant association between knowledge score and demographic variable age and no association was found with other selected variables. Conclusion: Hence this study is strongly recommended that development and implementing training and health education programme for students are essential for blood donation also further researches are needed.