Background: Needle stick injury as stated by United States National Institute of occupational safety and health, it is percutaneous wound caused by needle that accidently puncture the skin. Percutaneous injury and splashes of fluids have been recognized as source of exposure to blood borne pathogens such as hepatitis B, C virus and HIV for health care workers. Unsafe injection is one of the major risk factors in the occurrence of needle stick and other sharps related injuries in both HCWs and the general public. Objectives: Of the study were; To assess the pre-test knowledge score of staff nurses regarding needle stick injury, To assess the post-test knowledge score of staff nurses regarding needle stick injury, To assess the effectiveness of structured teaching program by comparing pre-test and post-test knowledge score of staff nurses regarding needle stick injury, To find out the association of the pre-test knowledge score of staff nurses regarding needle stick injury with their selected demographic variables [age, gender, education, religion, previous knowledge regarding needle stick injury, occupation of father and mother, education of father and mother, residence) Materials and Methods: A pre-experimental quantitative research approach design was used to conduct the study on sample of 40 nursing staff which were selected through purposive sampling technique at ibn sina hospital Budgam. The data was collected through self structured knowledge questionnaire was used for data collection which consists of two sections. Section 1 consists of Socio demographic data and section 11 consists of knowledge related to needle stick injury and further consists of three parts and total of 50 items. In total, the maximum score was 50 and minimum score was 0. Results: The findings of the study revealed that the mean post test knowledge score of the study subjects regarding needle stick injury which was significantly higher there is increase of knowledge after post test as compared to pretest knowledge score. Conclusion: Study concluded that knowledge of study subjects regarding needle stick injuries among staff nurses was inadequate, therefore structured teaching program was effective in enhancing their knowledge.Hence, it can be concluded that knowledge of study subjects after implementation of structured teaching program has increased.