Modification in Gene Expression of CCR5 Co-Receptor and Down Regulation of Virion Molecule with Primary Human T- cell in HIV Infection

Author: Dr. Bimal Singh, Dr. Sonalal Prasad Kushwaha, Tulsi Singh, Aakriti Gupta

Abstract:
About 1 2% of population in western European white people are resistant to hiv-1 infection due to modified gene of ccr5 receptor molecule. Modification of ccr5 receptor gene blocks the virion molecule to enter into the host cell. Mutations gene lead to slow progress rate of infection and low expression of cd4 + T cell. The hypothesis of researcher is that the modified allogeneic transplant with modified M303/M303 gene in ccr5 leads to resistant for HIV and AIDS patient. Deletion of gene in ccr5 receptor develops the ability of resistant with HIV-1. Down regulation of ccr5 receptor with macrophages T cell reduce the immune response and lead to slow progressive rate of infection. Different gene-editing methods are involved in ccr5 gene editing such as TALEN, ZFN, and CRISP/Cas. Mutant gene transplant in ccr5 receptor prevent the further exposure of dangerous retrovirus. As comparison of heterogeneous and homogenous .The heterogeneous analogues has more resistance as compare to homogenous genetic order. Chemokine and inflammatory mediator are the less active in heterogeneous. The homozygous mutation in ccr5 gene (ccr5∆32) base pair deletion induces nonfunctional ccr5 receptor and heterogonous mutation has resistant of HIV 1 and slow progressive effect